Calcium-binding protein in bull seminal vesicle secretion and seminal plasma

Constituents of Bull Seminal Plasma

Seminalplasmin, the major basic protein of bull seminal plasma, is a secretory protein of the seminal vesicles.

From the experimental results of three independent methods: (1) indirect immunofluorescence employing monospecific anti-seminalplasmin-IgGs, (2) cell-free translation of poly(A)+ RNA from seminal vesicle and testicular tissue, as well as (3) Northern analysis of poly(A)+ RNA of the latter tissues with a synthetic seminalplasmin-specific antisense DNA probe, it is concluded that the biosynthesis...

Adaptive evolution in rodent seminal vesicle secretion proteins.

Proteins involved in reproductive fitness have evolved unusually rapidly across diverse groups of organisms. These reproductive proteins show unusually high rates of amino acid substitutions, suggesting that the proteins have been subject to positive selection. We sought to identify seminal fluid proteins experiencing adaptive evolution because such proteins are often involved in sperm competit...

binding Proteins in Seminal Plasma Transcobalamin II

Study ofcobalamin-binding proteins revealed seminal plasma to be the most concentrated site of transcobalamin II in man. The next richest normal fluid, blood, has approximately one-tenth its concentration. Normal seminal unsaturated cobalamin-binding capacity averaged 15,030±7,290 pg/ml, of which 11,550±6,660 pg/ml was transcobalamin II. Transcobalamin II levels were markedly diminished in subj...

Correlations between seminal plasma enzyme activities and semen parameters in seminal fluid of Arabian horses

The objective of this study was to investigate aspartate-amino-transferase (AST), γ-glutamyl-transferase(GGT), alkaline phosphatase (ALP), lactate-dehydrogenase (LDH) and acid phosphatase (AcP) activities andsemen parameters (volume, pH, concentration, total sperm number (TSN), progressive motility, dead sperm,total morphological defect (TMD) and hypo-osmotic swelling test (HOST)) in seminal pl...